![]() In this proof-of-principle study, the preliminary experimental results suggest that the fiber-tip-based near-field FCS might be a good alternative to realize localized analysis at the single-molecule level. Related factors influencing the near-field FCS performance are investigated and discussed in detail. ![]() The detection volume under control of the SNOM system has been reduced over one order of magnitude compared to that of the conventional confocal FCS. Single-molecule FCS analysis at 100 nM Rhodamine 6G has been achieved by using bare chemically etched, tapered fiber tips. This near-field FCS is based on near-field illumination by coupling a scanning near-field optical microscope (SNOM) to a conventional confocal FCS. Lu, Guowei Lei, Franck H Angiboust, Jean-François Manfait, MichelĪ fiber-tip-based near-field fluorescence correlation spectroscopy (FCS) has been developed for confining the detection volume to sub-diffraction-limited dimensions. ![]() Confined detection volume of fluorescence correlation spectroscopy by bare fiber probes. ![]()
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